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All Journal Jurnal Akuakultur Indonesia Indonesian Aquaculture Journal Aquacultura Indonesiana

ALIM ISNANSETYO

Universitas Gadjah Mada

Author-ID : 289817

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences

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Growth, Total Production and Feed Efficiency of Catfish (Clarias sp.) Orally Administered with Shrimp Waste Hydrolyzate Hariyadi, Dimas Rizky; Isnansetyo, Alim; Istiqomah, Indah; Hardaningsih, Ign.; Wahyudi, Wahyudi; Kim, Sung Sam
Aquacultura Indonesiana Vol 19, No 1 (2018)
Publisher : Indonesian Aquaculture Society (MAI)

Shrimp Waste Hydrolyzate (SWH) /Shrimp Soluble Extract (SSE) is a product produced from the enzymatic process of shrimp waste. SWH contains essential and non-essential amino acids which useful for fish. This study aimed to determine effect of SWH on the growth and total production of catfish. The parameters observed were average growth rate, specific growth rate (SGR), food conversion ratio (FCR), protein efficiency ratio (PER), total production and length weight relationship from beginning until the end of rearing period. Growth performance was observed by calculating average growth rate and SGR of catfish. The results of this study showed that oral administration of SWH significantly increased SGR of catfish length at 4th sampling period with value 1.54% body length/day. Administration of SWH did not affect on the AGR, FCR, PER and total production. The growth pattern of catfish is negative allometric.

Oral Administration of Alginate From A Tropical Brown Seaweed, Sargassum sp. To Enhance Non-Spesific Defense In Walking Catfish (Clarias sp.) Isnansetyo, Alim; Irpani, Husni Mubarok; Wulansari, Tyas Ayu; Kasanah, Noer
Aquacultura Indonesiana Vol 15, No 1 (2014): Volume 15 Issue 1 Year 2014
Publisher : Indonesian Aquaculture Society (MAI)

Alginate that is usually extracted from brown seaweeds, is a bioactive substance with immunomodulator activity. The purpose of this research was to evaluate the effect of oral administration of alginate on the non-specific immune system of walking catfish (Clarias sp.). An experiment was conducted in a Completely Randomized Design with five treatments in triplicates. Alginate was supplemented in feed at doses of 0 (control treatment), 2, 4, 6 and 8 g/kg of feed. The walking catfish was fed twice daily at feeding rate of 5%. Non-specific defense parameters evaluated were Nitroblue Tetrazolium (NBT) activity, phagocytic activity (PA), phagocytic index (PI), leukocyte differentiation, hematocrit and leucocrit. The parameters were observed prior to be treated, 5th, 10th and 15th days after treatments. Oral administration of alginate at 4 g/kg of feed increased NBT and PA activities significantly (P<0.05) in 5 days, while alginate at a dose of 6 g/kg increased NBT activity and monocyte percentage significantly (P<0.05) in 5 days. These results suggested that oral administration of alginate at 4 g/kg effectively increased non-specific immune system of walking catfish.

Life Cycle of Marine Leech from Cultured Cantik Hybrid Grouper (Ephinephelus sp.) and Their Susceptibility Against Chemicals Murwantoko, Murwantoko; Condro, Sri Laksono; Isnansetyo, Alim; Zafran, Zafran
Aquacultura Indonesiana Vol 18, No 2 (2017)
Publisher : Indonesian Aquaculture Society (MAI)

Grouper is an important fish species due to high price both in domestic and international market. Several hybrid groupers have been released and can be accepted by farmers. A major production constraint in grouper culture is mortality due to disease. Leech is an ectoparasite for grouper which may cause significant loss, so that controlling this parasite is important as one of aquaculture management tools. The objectives of this study was to know the life cycle of leech on hybrid grouper and determine the efficacy of chemicals to kill leech under laboratory condition with diffferent dossage and immersing time. Life cycle was observed by reared an adult leech, counted the fecundity and observed the development of eggs until reach adult stages. The efficacy of formalin, albendazole, oxfendazole, levamisole, H2O2, CuSO4, ivermectin, vermizyn and freshwater on several concentration were assessed to kill adult leeches. All chemicals tested (except for freshwater), are dissolved in saline water salinity of 35 ppt at concentrations of 1000, 500, 250, 125 and 62.5 ppm. During 3 days of rearing, the adult leech could deposit of 11 eggs in average, with 600 µm – 800 µm in diameter. Twelve days were needed for the new egg inside the cocoon to hatch and develop into larvae under 24-25 °C at 34 ppt of salinity. It took another 9 days for the leeches larvae to grow reach mature stage. Five chemicals were able to kill leeches (Zeylanicobdela arugamensis), which were fresh water, formalin, levamisol, ivermectin, and CuSO4. Treatment by exposure leech to freshwater for 30 minutes shows effective to kill leech. Treatment with formalin with a dose of 250 ppm was able to kill leech after 1 hour immersion.

Short-term Response in Molecular and Biochemical Adaptation of White Shrimp (Litopenaeus vannamei) Postlarvae Reared in A Biofloc System Yasa, Ngurah Sedana; Anshory, Lutfi; Triastutik, Gemi; Murwantoko, Murwantoko; Isnansetyo, Alim; Lusiana, Lusiana
Aquacultura Indonesiana Vol 20, No 2 (2019)
Publisher : Indonesian Aquaculture Society (MAI)

Up regulation of heat shock proteins (HSPs) in organisms can be detected in response to many kinds of stressor. Recently there were few studies have focused on the three kinds of heat shock proteins (HSPs) and antioxidant enzyme activity after biofloc stress. The objective of this study was to investigate the effect of different biofloc volume on the expressions of (LvHSP60, LvHSP70, and LvHSP90), the activity of superoxide dismutase (SOD) and phenoloxidase (PO) of the L. vannamei after biofloc addition. PL10 L. vannamei were divided into three groups: precondition, biofloc stress (5mL/L, 10mL/L, 15mL/L), and recovery. The gene expression results showed that the expressional levels of LvHSP60, LvHSP70, and LvHSP90 were increased significantly in 6h treatment and tend return to normal conditions after 48h treatment. Superoxide dismutase activity was reduced during treatment and phenoloxidase activity was elevated slightly after 12h to 24h and tend to pretreatment level at recovery periods. All of these HSPs expression reverted to normal levels 6h after the recovery period. The results indicated that different expression patterns of the three HSPs. HSP60 have a longer and higher protection expression after 12h treatment than HSP70 and HSP90. HSP90 was more sensitive in 6h at all treatments than HSP60 and HSP70. It is concluded that supplementation of biofloc with the volume of 5-15mL/L caused Hsp protection in L. vannamei PLs at the first 6h to 48h treatment and increased phenoloxidase activity at 24-48h treatment and reduced survival rate of the white shrimp.

Co-Authors Aldi M. Halim Andi Niklani Angela Mariana Lusiastuti Anshory, Lutfi Condro, Sri Laksono Esti Handayani Hardi Gina Saptiani Hariyadi, Dimas Rizky Ign. Hardaningsih INDAH ISTIQOMAH Irpani, Husni Mubarok Kim, Sung Sam Lusiana, Lusiana Mira Mawardi Mukhlisnah Djalil Murwantoko . Noer Kasanah Rarastoeti Pratiwi Rina Puji Astuti Rudy Agung Nugroho SUWARNO HADISUSANTO Tito Arya Nugraha Triastutik, Gemi Triyanto Triyanto Wahyudi Wahyudi Widianingsih Rahayu Wulansari, Tyas Ayu Yasa, Ngurah Sedana Zafran, Zafran

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